The MTRD Head Office and Central Laboratories are located in Nairobi’s Industrial area, along Machakos Road. There are eight laboratories at the Headquarter; Bitumen, Soils, Concrete and Building materials, Chemistry, Paints, Physics, Foundation and Drilling, Traffic Survey and Pavement Monitoring Laboratories.
In addition to the Central laboratories, there are 16 regional laboratories located in Bungoma, Eldoret, Embu, Garissa, Kakamega, Kerugoya, Kisii, Kisumu, Machakos, Meru, Mombasa, Murang’a, Mwatate, Nakuru, Narok and Nyeri respectively.
The physical location and their email addresses are as indicated below;
| SNo | Laboratory | Physical Location | Contacts |
| 1 | Central | Along Machakos Road, Industrial Area, Nairobi | P.O BOX 11873-00400, Nairobi
Email: chiefengineer@mtrd.go.ke /info@mtrd.go.ke Telephone: +254 020 255 4950 |
| 2 | Mombasa | Along Shimanzi – Mahindi Road, Within Public Works Buildings Compound | P.O BOX 90663-80100, Mombasa |
| 3 | Mwatate | Along Voi – Mwatate Road, Next to Mwatate KeNHA Offices | P.O BOX 291-80300, Voi |
| 4 | Machakos | Along Kyumbi – Machakos Road, within Public Works Compound | P.O BOX 874-90100, Machakos |
| 5 | Embu | Along Embu – Kiritiri Road, within Public Works Compound | P.O BOX 485-60100, Embu |
| 6 | Meru | Off Meru – Mikinduri Road, Next to KURA Offices | P.O BOX 1357-60200, Meru |
| 7 | Kerugoya | Along Kerugoya – Baricho Road, next to KeRRA Offices | P.O BOX 390-10300, Kerugoya |
| 8 | Nyeri | Along Kaburini Road, next to Public Works Offices | P.O BOX 372-10100, Eldoret |
| 9 | Murang’a | Along Kaharati – Kangari Road, at the Old DC’s Office | P.O BOX 633-10200, Murang’a |
| 10 | Nakuru | Along Prison Road, Within Public Works compound | P.O BOX 19044-20100, Nakuru |
| 11 | Narok | Off Narok – Mau Narok road, opposite County Government offices | P.O BOX 398-20500, Narok |
| 12 | Kisii | Along Kisii – Keroka Road, next to KIHBT Kisii | P.O BOX 2254-40200, Kisii |
| 13 | Kisumu | Off Kisumu – Busia Road, next to KeNHA offices | P.O BOX 317-40100, Kisumu |
| 14 | Kakamega | Off Kisumu – Kakamega road, next to KeNHA offices | P.O BOX 14-50100, Kakamega |
| 15 | Bungoma | Along Kanduyi – Bungoma Road, within Public Works Compound | P.O BOX 14-50100, Bungoma |
| 16 | Eldoret | Along Oloo Street, within Public Works Compound | P.O BOX 2708-30100, Eldoret |
| 17 | Garissa | Along Kismayu Road, within Public Works Compound | P.O BOX 41-70100, Garissa |